Characterisation of a Putative Agent of Horizontal Gene Transfer Encoded by a Bacterial Prophage Region
Y3877831
1 Introduction
Double strand breaks (DSBs) are a form of DNA damage that results in the loss of bases on both strands of the DNA helix. They are commonly repaired by the non-homologous end-joining (NHEJ) pathway, which requires the protein Ku. Ku is a heterodimer of Ku70/Ku80 subunits. It identifies and binds to DSBs prior to NHEJ, and takes part in some transposition events (d’Adda di Fagagna, Weller, Doherty, et al., 2003). Homologues of Ku have been found in many bacteria. The most well- known homologue of Ku is the Gam protein of the Mu bacteriophage.
Mu is a temperate bacteriophage of Enterobacteria, particularly Escherichia coli. In bacteria, Mu acts as a transposable element (Fuller and Rice, 2017) – meaning it can change its position within the genome and replicate by forming two replication forks (Shapiro, 1979). The Mu transposon involves 3 proteins: MuA, MuB, and MuGam (d’Adda di Fagagna, Weller, Doherty, et al., 2003; Fuller and Rice, 2017). MuB polymerises adjacent to the E. coli target site and associates with MuA. MuA binds specific target sites and synapses them together to form the transposon. MuA remains bound until it is disassembled by the ATP-ClpX chaperone (Harshey, 2012). Meanwhile, MuGam binds to excised Mu phage DNA and protects it from digestion by host nucleases. Transposition events promote the uptake of foreign linear DNA: this function is known as natural competence and is linked to horizontal gene transfer (HGT). HGT is the transfer of foreign DNA between organisms and significantly contributes to the spread of antibiotic resistance and evolution in bacteria (Gyles and Boerlin, 2014).
MuGam enables natural competence in E. coli and is therefore a putative agent of HGT. Bacteria possessing a Gam-like prophage region should also be naturally competent[1]. In Haemophilus influenzae, Gam (HiGam) inhibits digestion of Mu phage DNA by nucleases (d’Adda di Fagagna, Weller, Doherty, et al., 2003). Through sequence alignment, we identified and cloned a putative homologue of Mu phage Gam from Haemophilus influenzae (HiGam). The structure – including binding affinity, oligomeric state – of HiGam was assessed in comparison to MuGam in literature. A range of assays were used to achieve this, namely electrophoretic mobility shift assay (EMSA), sodium dodecyl sulphate– polyacrylamide gel electrophoresis (SDS-PAGE) and size exclusion chromatography (SEC). Similarities to Mu will insinuate that HiGam will too function as an agent of horizontal gene transfer.
The aim of this report is to characterise HiGam by processing the data obtained from assays.
3 Preparing Data
4 Analysis
4.1 Polymerase Chain Reaction (PCR)
Diagnostic PCR was used to amplify the H. influenzae homologues, and to test for their transcription relative to the Genomic DNA (gDNA) control. Amplicon sizes were established using DNA ladder molecular weight (MW) standards. Results identified the transcription of HiA, HiB, HiGam, HiA-HiB and HiB-HiGam in H. influenzae. Figure @(fig:pcr-fig) shows
\[\Large \color{#8D9FCA} y \color{black} = \color{#E789C2} m \color{#66c2a4} x \color{black} + \color{#FFD82F} c\]
Expressed Amplicons
PCR Dataset
| Library | Amplicon | Distance (cm) | Expected Size (bp) | Relative Front (Rf) | Log(Rf) | Actual Size (bp) | Size Difference (bp) |
|---|---|---|---|---|---|---|---|
| gDNA | HiA | 11.40 | 157 | 0.09 | 2.01 | 102.19 | 34.91 |
| HiB | 11.04 | 208 | 0.12 | 2.09 | 122.42 | 41.15 | |
| HiGam | 10.77 | 245 | 0.14 | 2.15 | 140.17 | 42.79 | |
| HiA-HiB | 5.27 | 2122 | 0.58 | 3.34 | 2213.08 | -4.29 | |
| HiB-HiGam | 6.48 | 1319 | 0.48 | 3.08 | 1206.04 | 8.56 | |
| cDNA | HiA | 11.77 | 157 | 0.06 | 2.00 | 100.80 | 35.80 |
| HiB | 11.42 | 208 | 0.09 | 2.08 | 119.90 | 42.35 | |
| HiGam | 10.14 | 245 | 0.19 | 2.35 | 226.17 | 7.69 | |
| HiA-HiB | 5.22 | 2122 | 0.58 | 3.41 | 2593.09 | -22.20 | |
| HiB-HiGam | 6.46 | 1319 | 0.48 | 3.15 | 1402.23 | -6.31 |
4.2 Electrophoretic Mobility Shift Assay (EMSA) & Binding Affinity
EMSA
Electrophoretic mobility shift assay (EMSA) was used to examine whether Im9-HiGam–DNA had an affinity for closed circular DNA or linear DNA. Im9-HiGam was observed to bind preferentially to linear DNA with a KD of approximately 0.52 (4.1). Results estimate Im9-HiGam has a binding dimeric footprint of base pairs.
EMSA
Figure 4.1: (fig:emsa-fig)
Dataset
| Concentration | Distance | Relative Front | Residuals |
|---|---|---|---|
| 0.0 | 6.20 | 0.31 | 0.01 |
| 0.5 | 4.81 | 0.46 | -0.06 |
| 1.0 | 3.20 | 0.64 | 0.05 |
| 1.5 | 3.10 | 0.66 | 0.02 |
| 2.0 | 3.00 | 0.67 | 0.01 |
| 3.0 | 3.00 | 0.67 | -0.01 |
| 4.0 | 3.00 | 0.67 | -0.03 |
4.3 Size Exclusion Chromatography (SEC)
Size exclusion chromatography (SEC) was used to explore the molecular weight and oligomeric state of Im9-HiGam. 7 chromatograms of known MW were plotted and a linear regression was performed to determine the relationship between elution volume and molecular weight. The SEC data showed a peak for both wavelengths, with a MW of approximately 77703.42 Daltons for A254 and 58853.86 Daltons for A280. This gives Im9-HiGam has an estimated dimeric footprint of approximately 28.56 base pairs.
SEC Analysis
Calibration Plot
SEC Standards
SEC Dataset
| Volume | a254 | a280 |
|---|---|---|
| 0.00 | 0.10 | 0.18 |
| 0.02 | 0.20 | 0.18 |
| 0.05 | 0.07 | -0.01 |
| 0.07 | -0.04 | -0.04 |
| 0.10 | -0.02 | -0.20 |
| 0.12 | -0.11 | 0.10 |
| 0.14 | 0.07 | -0.09 |
| 0.17 | -0.03 | 0.06 |
| 0.19 | -0.06 | -0.36 |
| 0.22 | 0.07 | -0.15 |
| 0.24 | 0.14 | -0.33 |
| 0.26 | -0.12 | -0.10 |
| 0.29 | 0.19 | 0.14 |
| 0.31 | 0.02 | 0.04 |
| 0.34 | 0.05 | 0.12 |
| 0.36 | 0.03 | 0.19 |
| 0.38 | -0.02 | -0.32 |
| 0.41 | 0.12 | 0.21 |
| 0.43 | -0.13 | 0.01 |
| 0.46 | 0.08 | -0.07 |
| 0.48 | -0.11 | 0.15 |
| 0.50 | -0.06 | -0.03 |
| 0.53 | -0.11 | -0.02 |
| 0.55 | 0.01 | -0.02 |
| 0.58 | -0.15 | -0.04 |
| 0.60 | 0.10 | -0.03 |
| 0.62 | 0.13 | -0.12 |
| 0.65 | -0.17 | 0.32 |
| 0.67 | 0.05 | 0.04 |
| 0.70 | 0.19 | 0.27 |
| 0.72 | 0.12 | -0.21 |
| 0.74 | 0.20 | -0.25 |
| 0.77 | 0.08 | 0.06 |
| 0.79 | -0.14 | 0.18 |
| 0.82 | -0.03 | 0.04 |
| 0.84 | -0.17 | -0.13 |
| 0.86 | 0.10 | 0.08 |
| 0.89 | 0.07 | -0.04 |
| 0.91 | 0.02 | 0.35 |
| 0.94 | 0.16 | -0.02 |
| 0.96 | 0.00 | 0.09 |
| 0.98 | 0.03 | 0.01 |
| 1.01 | -0.10 | 0.12 |
| 1.03 | -0.16 | -0.23 |
| 1.06 | 0.13 | -0.09 |
| 1.08 | -0.19 | -0.04 |
| 1.11 | 0.11 | -0.14 |
| 1.13 | 0.03 | 0.00 |
| 1.15 | 0.04 | -0.05 |
| 1.18 | -0.06 | 0.01 |
| 1.20 | -0.18 | 0.02 |
| 1.23 | 0.05 | 0.15 |
| 1.25 | 0.01 | 0.02 |
| 1.27 | -0.14 | -0.27 |
| 1.30 | 0.15 | -0.19 |
| 1.32 | -0.17 | -0.29 |
| 1.35 | -0.02 | -0.18 |
| 1.37 | 0.14 | -0.15 |
| 1.39 | 0.02 | 0.10 |
| 1.42 | -0.06 | -0.27 |
| 1.44 | 0.01 | 0.03 |
| 1.47 | -0.13 | 0.05 |
| 1.49 | 0.07 | -0.32 |
| 1.51 | -0.07 | -0.21 |
| 1.54 | -0.15 | -0.04 |
| 1.56 | 0.01 | -0.02 |
| 1.59 | -0.01 | 0.11 |
| 1.61 | -0.13 | -0.16 |
| 1.63 | 0.03 | 0.19 |
| 1.66 | 0.13 | 0.19 |
| 1.68 | -0.18 | -0.09 |
| 1.71 | 0.03 | 0.07 |
| 1.73 | 0.15 | -0.02 |
| 1.75 | 0.03 | 0.14 |
| 1.78 | -0.01 | 0.18 |
| 1.80 | 0.14 | -0.19 |
| 1.83 | 0.11 | 0.26 |
| 1.85 | -0.15 | -0.14 |
| 1.87 | -0.17 | -0.24 |
| 1.90 | 0.11 | -0.03 |
| 1.92 | -0.21 | 0.17 |
| 1.95 | 0.12 | 0.29 |
| 1.97 | 0.06 | -0.28 |
| 1.99 | 0.19 | -0.03 |
| 2.02 | 0.15 | -0.06 |
| 2.04 | -0.10 | 0.21 |
| 2.07 | -0.21 | -0.19 |
| 2.09 | 0.06 | 0.08 |
| 2.11 | 0.05 | -0.02 |
| 2.14 | 0.17 | -0.34 |
| 2.16 | -0.13 | -0.24 |
| 2.19 | 0.17 | 0.10 |
| 2.21 | -0.10 | 0.08 |
| 2.23 | 0.06 | -0.04 |
| 2.26 | -0.14 | 0.00 |
| 2.28 | 0.09 | 0.10 |
| 2.31 | -0.04 | 0.18 |
| 2.33 | 0.03 | 0.12 |
| 2.35 | -0.03 | 0.21 |
| 2.38 | -0.19 | 0.06 |
| 2.40 | 0.19 | 0.33 |
| 2.43 | -0.06 | 0.24 |
| 2.45 | 0.12 | 0.28 |
| 2.47 | -0.12 | 0.07 |
| 2.50 | 0.16 | 0.19 |
| 2.52 | -0.14 | 0.21 |
| 2.55 | 0.17 | 0.02 |
| 2.57 | -0.07 | 0.12 |
| 2.59 | 0.17 | 0.01 |
| 2.62 | 0.14 | -0.06 |
| 2.64 | 0.10 | 0.18 |
| 2.67 | -0.05 | 0.17 |
| 2.69 | 0.10 | 0.03 |
| 2.71 | 0.11 | 0.24 |
| 2.74 | -0.10 | -0.08 |
| 2.76 | 0.03 | -0.03 |
| 2.79 | 0.05 | -0.01 |
| 2.81 | -0.13 | 0.33 |
| 2.83 | 0.08 | 0.03 |
| 2.86 | 0.15 | -0.02 |
| 2.88 | -0.02 | 0.32 |
| 2.91 | -0.20 | 0.35 |
| 2.93 | 0.00 | -0.07 |
| 2.95 | 0.07 | 0.18 |
| 2.98 | -0.12 | 0.06 |
| 3.00 | -0.01 | 0.10 |
| 3.03 | -0.03 | 0.06 |
| 3.05 | -0.14 | -0.16 |
| 3.08 | -0.02 | 0.13 |
| 3.10 | 0.00 | -0.11 |
| 3.12 | -0.10 | -0.20 |
| 3.15 | -0.13 | -0.07 |
| 3.17 | 0.02 | -0.13 |
| 3.20 | 0.13 | 0.21 |
| 3.22 | 0.19 | -0.18 |
| 3.24 | 0.14 | -0.08 |
| 3.27 | 0.18 | 0.24 |
| 3.29 | -0.16 | -0.31 |
| 3.32 | 0.05 | -0.23 |
| 3.34 | -0.02 | 0.05 |
| 3.36 | -0.02 | -0.11 |
| 3.39 | -0.02 | 0.12 |
| 3.41 | 0.02 | -0.21 |
| 3.44 | 0.04 | 0.12 |
| 3.46 | -0.10 | 0.01 |
| 3.48 | 0.01 | 0.03 |
| 3.51 | 0.09 | 0.11 |
| 3.53 | 0.09 | -0.03 |
| 3.56 | 0.12 | -0.08 |
| 3.58 | 0.19 | -0.06 |
| 3.60 | 0.10 | 0.06 |
| 3.63 | -0.01 | -0.12 |
| 3.65 | 0.19 | -0.02 |
| 3.68 | -0.11 | -0.16 |
| 3.70 | -0.06 | -0.08 |
| 3.72 | -0.03 | -0.39 |
| 3.75 | 0.00 | -0.11 |
| 3.77 | -0.07 | 0.00 |
| 3.80 | 0.11 | -0.16 |
| 3.82 | 0.13 | -0.07 |
| 3.84 | 0.10 | -0.22 |
| 3.87 | -0.16 | -0.20 |
| 3.89 | 0.16 | -0.08 |
| 3.92 | 0.06 | 0.32 |
| 3.94 | 0.11 | 0.13 |
| 3.96 | -0.05 | -0.12 |
| 3.99 | -0.17 | -0.18 |
| 4.01 | 0.11 | -0.04 |
| 4.04 | -0.02 | -0.31 |
| 4.06 | -0.05 | -0.02 |
| 4.08 | 0.11 | 0.02 |
| 4.11 | 0.13 | -0.32 |
| 4.13 | 0.15 | -0.16 |
| 4.16 | 0.11 | -0.28 |
| 4.18 | 0.03 | -0.03 |
| 4.20 | 0.06 | 0.06 |
| 4.23 | -0.01 | 0.15 |
| 4.25 | -0.03 | 0.16 |
| 4.28 | -0.01 | 0.15 |
| 4.30 | -0.15 | 0.07 |
| 4.32 | 0.20 | -0.01 |
| 4.35 | -0.15 | -0.15 |
| 4.37 | -0.02 | 0.12 |
| 4.40 | 0.16 | 0.10 |
| 4.42 | -0.13 | -0.23 |
| 4.44 | -0.05 | -0.13 |
| 4.47 | -0.15 | -0.29 |
| 4.49 | -0.03 | 0.03 |
| 4.52 | -0.01 | 0.07 |
| 4.54 | -0.03 | -0.19 |
| 4.56 | 0.12 | -0.04 |
| 4.59 | -0.20 | -0.01 |
| 4.61 | 0.12 | 0.27 |
| 4.64 | 0.04 | -0.21 |
| 4.66 | -0.12 | -0.18 |
| 4.68 | 0.03 | -0.15 |
| 4.71 | -0.08 | -0.05 |
| 4.73 | -0.06 | 0.09 |
| 4.76 | -0.06 | 0.12 |
| 4.78 | -0.19 | -0.01 |
| 4.80 | 0.02 | -0.35 |
| 4.83 | 0.02 | 0.33 |
| 4.85 | 0.09 | 0.25 |
| 4.88 | -0.14 | -0.10 |
| 4.90 | 0.15 | 0.18 |
| 4.92 | 0.15 | 0.12 |
| 4.95 | -0.10 | 0.02 |
| 4.97 | -0.03 | -0.07 |
| 5.00 | -0.05 | -0.01 |
| 5.02 | -0.13 | -0.03 |
| 5.05 | 0.04 | 0.23 |
| 5.07 | 0.09 | -0.07 |
| 5.09 | 0.09 | -0.06 |
| 5.12 | 0.02 | 0.04 |
| 5.14 | -0.15 | -0.15 |
| 5.17 | -0.11 | 0.02 |
| 5.19 | -0.03 | 0.31 |
| 5.21 | -0.20 | 0.01 |
| 5.24 | 0.06 | -0.15 |
| 5.26 | 0.00 | -0.24 |
| 5.29 | 0.09 | 0.21 |
| 5.31 | -0.08 | 0.05 |
| 5.33 | 0.07 | -0.12 |
| 5.36 | 0.11 | 0.09 |
| 5.38 | -0.12 | -0.06 |
| 5.41 | -0.03 | -0.14 |
| 5.43 | -0.05 | -0.03 |
| 5.45 | 0.13 | -0.18 |
| 5.48 | -0.06 | -0.03 |
| 5.50 | 0.20 | 0.15 |
| 5.53 | -0.05 | 0.20 |
| 5.55 | 0.05 | -0.08 |
| 5.57 | 0.14 | 0.05 |
| 5.60 | 0.06 | -0.12 |
| 5.62 | -0.12 | 0.18 |
| 5.65 | -0.16 | 0.01 |
| 5.67 | -0.11 | -0.20 |
| 5.69 | -0.16 | -0.28 |
| 5.72 | -0.11 | 0.06 |
| 5.74 | -0.16 | -0.20 |
| 5.77 | -0.07 | -0.07 |
| 5.79 | 0.01 | -0.01 |
| 5.81 | 0.16 | -0.07 |
| 5.84 | -0.12 | 0.06 |
| 5.86 | 0.12 | 0.12 |
| 5.89 | -0.07 | 0.19 |
| 5.91 | 0.02 | 0.27 |
| 5.93 | -0.10 | -0.02 |
| 5.96 | -0.12 | -0.05 |
| 5.98 | 0.18 | 0.28 |
| 6.01 | -0.15 | 0.01 |
| 6.03 | 0.12 | -0.07 |
| 6.05 | -0.16 | 0.24 |
| 6.08 | 0.02 | 0.06 |
| 6.10 | -0.01 | 0.05 |
| 6.13 | -0.08 | 0.25 |
| 6.15 | 0.09 | 0.24 |
| 6.17 | -0.01 | 0.27 |
| 6.20 | -0.03 | -0.16 |
| 6.22 | 0.07 | -0.32 |
| 6.25 | -0.19 | -0.04 |
| 6.27 | -0.19 | -0.22 |
| 6.29 | -0.08 | 0.22 |
| 6.32 | 0.17 | -0.16 |
| 6.34 | -0.08 | 0.01 |
| 6.37 | -0.11 | -0.30 |
| 6.39 | -0.12 | 0.03 |
| 6.41 | 0.03 | -0.29 |
| 6.44 | -0.12 | 0.31 |
| 6.46 | -0.08 | 0.01 |
| 6.49 | 0.13 | 0.21 |
| 6.51 | 0.05 | -0.06 |
| 6.53 | 0.16 | -0.06 |
| 6.56 | -0.13 | -0.12 |
| 6.58 | 0.17 | 0.33 |
| 6.61 | 0.13 | 0.00 |
| 6.63 | 0.12 | -0.09 |
| 6.65 | -0.18 | -0.06 |
| 6.68 | 0.21 | 0.33 |
| 6.70 | 0.12 | 0.31 |
| 6.73 | -0.11 | 0.00 |
| 6.75 | -0.17 | 0.00 |
| 6.77 | 0.16 | 0.23 |
| 6.80 | -0.03 | -0.05 |
| 6.82 | -0.09 | 0.27 |
| 6.85 | 0.18 | 0.01 |
| 6.87 | 0.17 | -0.27 |
| 6.89 | -0.09 | 0.11 |
| 6.92 | 0.01 | -0.17 |
| 6.94 | -0.17 | -0.02 |
| 6.97 | 0.19 | 0.01 |
| 6.99 | -0.06 | 0.29 |
| 7.02 | 0.01 | 0.04 |
| 7.04 | 0.11 | -0.17 |
| 7.06 | 0.02 | -0.33 |
| 7.09 | -0.01 | -0.15 |
| 7.11 | -0.07 | -0.30 |
| 7.14 | -0.05 | -0.26 |
| 7.16 | -0.05 | -0.04 |
| 7.18 | -0.12 | -0.17 |
| 7.21 | 0.14 | -0.10 |
| 7.23 | -0.08 | -0.08 |
| 7.26 | -0.16 | 0.21 |
| 7.28 | -0.03 | -0.12 |
| 7.30 | -0.16 | 0.13 |
| 7.33 | 0.18 | 0.12 |
| 7.35 | -0.19 | 0.14 |
| 7.38 | 0.00 | 0.17 |
| 7.40 | 0.08 | 0.23 |
| 7.42 | -0.18 | 0.14 |
| 7.45 | -0.03 | -0.22 |
| 7.47 | 0.04 | 0.21 |
| 7.50 | -0.04 | 0.07 |
| 7.52 | -0.14 | -0.08 |
| 7.54 | -0.08 | 0.05 |
| 7.57 | 0.16 | 0.02 |
| 7.59 | 0.04 | -0.08 |
| 7.62 | 0.01 | 0.27 |
| 7.64 | -0.17 | -0.05 |
| 7.66 | 0.06 | 0.24 |
| 7.69 | 0.05 | -0.05 |
| 7.71 | 0.15 | -0.05 |
| 7.74 | -0.11 | 0.03 |
| 7.76 | 0.17 | 0.22 |
| 7.78 | -0.18 | 0.31 |
| 7.81 | -0.04 | 0.25 |
| 7.83 | -0.08 | -0.34 |
| 7.86 | -0.19 | -0.37 |
| 7.88 | -0.08 | -0.05 |
| 7.90 | 0.17 | 0.11 |
| 7.93 | -0.20 | 0.03 |
| 7.95 | -0.01 | 0.22 |
| 7.98 | 0.18 | -0.14 |
| 8.00 | -0.12 | -0.06 |
| 8.02 | 0.08 | -0.31 |
| 8.05 | -0.04 | -0.23 |
| 8.07 | 0.16 | 0.01 |
| 8.10 | -0.18 | 0.17 |
| 8.12 | -0.16 | -0.13 |
| 8.14 | 0.06 | 0.21 |
| 8.17 | 0.08 | -0.13 |
| 8.19 | -0.04 | 0.01 |
| 8.22 | -0.04 | -0.16 |
| 8.24 | 0.15 | -0.15 |
| 8.26 | -0.18 | -0.15 |
| 8.29 | 0.06 | -0.05 |
| 8.31 | -0.21 | 0.24 |
| 8.34 | 0.12 | 0.03 |
| 8.36 | 0.02 | -0.22 |
| 8.38 | 0.10 | 0.06 |
| 8.41 | -0.09 | 0.28 |
| 8.43 | -0.18 | -0.04 |
| 8.46 | 0.03 | -0.05 |
| 8.48 | 0.06 | -0.18 |
| 8.50 | 0.10 | 0.07 |
| 8.53 | 0.08 | 0.31 |
| 8.55 | -0.03 | 0.06 |
| 8.58 | 0.19 | 0.08 |
| 8.60 | -0.08 | 0.01 |
| 8.62 | -0.03 | -0.17 |
| 8.65 | -0.10 | -0.12 |
| 8.67 | -0.17 | 0.31 |
| 8.70 | -0.13 | -0.17 |
| 8.72 | 0.07 | 0.22 |
| 8.74 | -0.08 | 0.20 |
| 8.77 | 0.22 | 0.10 |
| 8.79 | 0.12 | -0.21 |
| 8.82 | -0.11 | 0.07 |
| 8.84 | -0.02 | 0.24 |
| 8.86 | -0.19 | 0.18 |
| 8.89 | 0.16 | -0.08 |
| 8.91 | 0.02 | -0.13 |
| 8.94 | 0.17 | 0.24 |
| 8.96 | -0.12 | 0.16 |
| 8.98 | -0.10 | 0.13 |
| 9.01 | -0.12 | 0.13 |
| 9.03 | 0.01 | -0.09 |
| 9.06 | -0.02 | 0.15 |
| 9.08 | -0.10 | 0.25 |
| 9.11 | -0.06 | 0.08 |
| 9.13 | -0.01 | -0.08 |
| 9.15 | 0.05 | -0.07 |
| 9.18 | 0.16 | 0.04 |
| 9.20 | 0.11 | -0.36 |
| 9.23 | 0.05 | 0.01 |
| 9.25 | -0.10 | -0.09 |
| 9.27 | 0.15 | -0.06 |
| 9.30 | -0.19 | -0.15 |
| 9.32 | -0.10 | 0.21 |
| 9.35 | -0.13 | -0.07 |
| 9.37 | 0.06 | -0.21 |
| 9.39 | 0.09 | -0.03 |
| 9.42 | 0.19 | 0.04 |
| 9.44 | 0.15 | -0.18 |
| 9.47 | -0.19 | 0.03 |
| 9.49 | 0.13 | -0.01 |
| 9.51 | 0.09 | -0.33 |
| 9.54 | 0.14 | 0.02 |
| 9.56 | 0.17 | 0.05 |
| 9.59 | 0.01 | -0.17 |
| 9.61 | 0.01 | -0.26 |
| 9.63 | -0.09 | 0.08 |
| 9.66 | -0.03 | 0.16 |
| 9.68 | -0.06 | -0.02 |
| 9.71 | 0.07 | -0.01 |
| 9.73 | -0.04 | -0.15 |
| 9.75 | 0.05 | 0.01 |
| 9.78 | -0.10 | -0.14 |
| 9.80 | 0.00 | -0.15 |
| 9.83 | 0.02 | -0.05 |
| 9.85 | 0.10 | 0.06 |
| 9.87 | -0.07 | 0.25 |
| 9.90 | -0.01 | 0.02 |
| 9.92 | 0.11 | -0.01 |
| 9.95 | -0.16 | 0.01 |
| 9.97 | -0.09 | -0.03 |
| 9.99 | 0.18 | -0.21 |
| 10.02 | -0.01 | -0.05 |
| 10.04 | 0.18 | 0.08 |
| 10.07 | 0.04 | 0.11 |
| 10.09 | -0.12 | 0.05 |
| 10.11 | -0.05 | 0.04 |
| 10.14 | -0.13 | -0.30 |
| 10.16 | 0.08 | -0.30 |
| 10.19 | 0.06 | -0.33 |
| 10.21 | 0.00 | -0.12 |
| 10.23 | 0.16 | -0.02 |
| 10.26 | 0.04 | 0.22 |
| 10.28 | -0.18 | -0.24 |
| 10.31 | 0.00 | -0.06 |
| 10.33 | 0.03 | -0.16 |
| 10.35 | -0.13 | 0.18 |
| 10.38 | 0.14 | 0.28 |
| 10.40 | -0.10 | 0.04 |
| 10.43 | -0.08 | 0.37 |
| 10.45 | 0.15 | 0.20 |
| 10.47 | 0.00 | 0.00 |
| 10.50 | 0.01 | -0.37 |
| 10.52 | -0.17 | -0.14 |
| 10.55 | 0.10 | 0.13 |
| 10.57 | 0.14 | -0.01 |
| 10.59 | 0.06 | -0.30 |
| 10.62 | 0.14 | -0.20 |
| 10.64 | 0.09 | -0.26 |
| 10.67 | -0.02 | 0.31 |
| 10.69 | 0.06 | -0.29 |
| 10.71 | -0.04 | 0.06 |
| 10.74 | 0.17 | 0.05 |
| 10.76 | -0.10 | 0.22 |
| 10.79 | 0.15 | 0.03 |
| 10.81 | 0.08 | 0.18 |
| 10.83 | 0.20 | -0.02 |
| 10.86 | 0.05 | -0.23 |
| 10.88 | -0.11 | -0.01 |
| 10.91 | -0.10 | -0.27 |
| 10.93 | 0.06 | -0.22 |
| 10.95 | -0.07 | -0.34 |
| 10.98 | 0.07 | -0.30 |
| 11.00 | -0.16 | 0.17 |
| 11.03 | 0.15 | 0.03 |
| 11.05 | -0.19 | -0.06 |
| 11.08 | -0.09 | 0.00 |
| 11.10 | 0.05 | -0.13 |
| 11.12 | -0.04 | 0.10 |
| 11.15 | 0.12 | 0.07 |
| 11.17 | -0.15 | 0.08 |
| 11.20 | 0.08 | 0.16 |
| 11.22 | 0.18 | -0.29 |
| 11.24 | -0.13 | 0.29 |
| 11.27 | 0.00 | 0.35 |
| 11.29 | 0.14 | 0.15 |
| 11.32 | 0.17 | 0.11 |
| 11.34 | 0.15 | -0.15 |
| 11.36 | -0.04 | 0.12 |
| 11.39 | 0.04 | -0.15 |
| 11.41 | 0.02 | 0.03 |
| 11.44 | -0.09 | 0.09 |
| 11.46 | -0.19 | 0.25 |
| 11.48 | 0.02 | -0.25 |
| 11.51 | 0.08 | 0.15 |
| 11.53 | 0.14 | -0.04 |
| 11.56 | -0.04 | -0.19 |
| 11.58 | 0.04 | -0.17 |
| 11.60 | -0.08 | -0.08 |
| 11.63 | 0.03 | -0.09 |
| 11.65 | -0.14 | 0.02 |
| 11.68 | 0.01 | 0.02 |
| 11.70 | 0.09 | 0.17 |
| 11.72 | 0.04 | 0.01 |
| 11.75 | 0.18 | 0.16 |
| 11.77 | -0.07 | 0.12 |
| 11.80 | -0.14 | 0.03 |
| 11.82 | -0.09 | -0.12 |
| 11.84 | 0.05 | -0.20 |
| 11.87 | -0.02 | -0.04 |
| 11.89 | 0.12 | -0.06 |
| 11.92 | -0.18 | 0.11 |
| 11.94 | -0.07 | -0.04 |
| 11.96 | -0.05 | -0.04 |
| 11.99 | 0.06 | -0.16 |
| 12.01 | 0.05 | 0.28 |
| 12.04 | 0.21 | -0.04 |
| 12.06 | -0.03 | 0.23 |
| 12.08 | 0.19 | 0.03 |
| 12.11 | 0.14 | 0.20 |
| 12.13 | -0.01 | 0.11 |
| 12.16 | -0.05 | 0.07 |
| 12.18 | 0.13 | -0.08 |
| 12.20 | 0.10 | -0.01 |
| 12.23 | 0.10 | -0.03 |
| 12.25 | -0.17 | -0.21 |
| 12.28 | -0.19 | -0.25 |
| 12.30 | -0.05 | -0.33 |
| 12.32 | 0.03 | -0.24 |
| 12.35 | 0.04 | -0.08 |
| 12.37 | -0.01 | 0.01 |
| 12.40 | -0.02 | 0.06 |
| 12.42 | -0.01 | 0.01 |
| 12.44 | -0.03 | -0.07 |
| 12.47 | -0.12 | -0.02 |
| 12.49 | 0.16 | 0.18 |
| 12.52 | 0.08 | 0.05 |
| 12.54 | -0.01 | -0.08 |
| 12.56 | 0.21 | -0.15 |
| 12.59 | -0.01 | 0.01 |
| 12.61 | -0.12 | -0.11 |
| 12.64 | -0.09 | 0.09 |
| 12.66 | -0.19 | 0.21 |
| 12.68 | 0.11 | 0.04 |
| 12.71 | -0.09 | -0.01 |
| 12.73 | 0.11 | -0.29 |
| 12.76 | 0.19 | 0.03 |
| 12.78 | 0.04 | 0.09 |
| 12.80 | 0.19 | -0.02 |
| 12.83 | 0.13 | 0.02 |
| 12.85 | 0.08 | 0.09 |
| 12.88 | -0.01 | 0.12 |
| 12.90 | -0.07 | 0.05 |
| 12.92 | 0.13 | -0.12 |
| 12.95 | -0.12 | -0.18 |
| 12.97 | 0.08 | 0.25 |
| 13.00 | 0.00 | -0.16 |
| 13.02 | -0.01 | -0.13 |
| 13.05 | -0.12 | 0.27 |
| 13.07 | -0.16 | 0.07 |
| 13.09 | -0.03 | 0.13 |
| 13.12 | -0.14 | 0.21 |
| 13.14 | -0.07 | 0.09 |
| 13.17 | 0.07 | 0.33 |
| 13.19 | 0.04 | 0.12 |
| 13.21 | -0.10 | -0.23 |
| 13.24 | -0.14 | 0.29 |
| 13.26 | 0.04 | 0.12 |
| 13.29 | 0.07 | 0.12 |
| 13.31 | 0.10 | 0.29 |
| 13.33 | -0.09 | 0.11 |
| 13.36 | -0.17 | 0.13 |
| 13.38 | 0.11 | -0.26 |
| 13.41 | -0.12 | -0.03 |
| 13.43 | -0.03 | 0.00 |
| 13.45 | 0.14 | 0.04 |
| 13.48 | 0.11 | -0.22 |
| 13.50 | 0.13 | 0.27 |
| 13.53 | -0.04 | 0.10 |
| 13.55 | -0.13 | -0.22 |
| 13.57 | 0.19 | -0.10 |
| 13.60 | -0.16 | 0.05 |
| 13.62 | 0.10 | 0.17 |
| 13.65 | 0.10 | -0.16 |
| 13.67 | 0.11 | 0.21 |
| 13.69 | -0.18 | 0.11 |
| 13.72 | 0.17 | 0.05 |
| 13.74 | 0.17 | 0.38 |
| 13.77 | 0.03 | -0.17 |
| 13.79 | 0.20 | 0.08 |
| 13.81 | -0.17 | 0.07 |
| 13.84 | 0.15 | -0.20 |
| 13.86 | -0.12 | 0.16 |
| 13.89 | 0.00 | -0.22 |
| 13.91 | 0.19 | -0.14 |
| 13.93 | -0.12 | 0.15 |
| 13.96 | 0.07 | -0.31 |
| 13.98 | 0.02 | 0.07 |
| 14.01 | 0.10 | 0.10 |
| 14.03 | 0.00 | 0.24 |
| 14.05 | 0.18 | -0.39 |
| 14.08 | -0.08 | 0.32 |
| 14.10 | -0.04 | -0.11 |
| 14.13 | -0.15 | -0.17 |
| 14.15 | -0.18 | -0.26 |
| 14.17 | 0.15 | -0.11 |
| 14.20 | 0.00 | 0.23 |
| 14.22 | -0.19 | 0.39 |
| 14.25 | -0.14 | 0.12 |
| 14.27 | 0.13 | 0.37 |
| 14.29 | 0.01 | 0.47 |
| 14.32 | -0.09 | 0.85 |
| 14.34 | 0.33 | 0.97 |
| 14.37 | 0.18 | 1.73 |
| 14.39 | 0.65 | 2.07 |
| 14.41 | 0.92 | 2.81 |
| 14.44 | 1.61 | 3.61 |
| 14.46 | 2.56 | 4.83 |
| 14.49 | 3.95 | 5.86 |
| 14.51 | 5.78 | 7.80 |
| 14.53 | 8.70 | 9.62 |
| 14.56 | 12.19 | 11.65 |
| 14.58 | 17.27 | 13.71 |
| 14.61 | 23.22 | 16.31 |
| 14.63 | 30.75 | 19.22 |
| 14.65 | 39.16 | 21.21 |
| 14.68 | 48.40 | 24.35 |
| 14.70 | 58.13 | 26.80 |
| 14.73 | 67.83 | 28.88 |
| 14.75 | 76.94 | 31.51 |
| 14.77 | 84.94 | 32.86 |
| 14.80 | 90.78 | 33.98 |
| 14.82 | 94.07 | 34.83 |
| 14.85 | 95.20 | 34.81 |
| 14.87 | 93.11 | 34.84 |
| 14.89 | 88.61 | 34.25 |
| 14.92 | 81.84 | 32.51 |
| 14.94 | 73.54 | 31.18 |
| 14.97 | 64.28 | 29.91 |
| 14.99 | 54.65 | 28.31 |
| 15.02 | 45.39 | 27.15 |
| 15.04 | 36.74 | 26.37 |
| 15.06 | 29.31 | 26.30 |
| 15.09 | 23.31 | 27.59 |
| 15.11 | 18.60 | 29.29 |
| 15.14 | 14.85 | 32.40 |
| 15.16 | 12.24 | 37.32 |
| 15.18 | 10.72 | 42.31 |
| 15.21 | 9.92 | 48.80 |
| 15.23 | 9.85 | 55.12 |
| 15.26 | 9.50 | 61.48 |
| 15.28 | 9.94 | 67.53 |
| 15.30 | 9.92 | 72.08 |
| 15.33 | 9.95 | 75.20 |
| 15.35 | 10.25 | 76.03 |
| 15.38 | 10.08 | 75.04 |
| 15.40 | 9.57 | 71.51 |
| 15.42 | 9.36 | 66.80 |
| 15.45 | 8.75 | 60.38 |
| 15.47 | 8.00 | 53.11 |
| 15.50 | 7.17 | 45.22 |
| 15.52 | 6.24 | 37.54 |
| 15.54 | 5.53 | 29.93 |
| 15.57 | 4.41 | 23.39 |
| 15.59 | 3.64 | 17.80 |
| 15.62 | 3.11 | 12.88 |
| 15.64 | 2.57 | 9.75 |
| 15.66 | 2.00 | 6.77 |
| 15.69 | 1.39 | 4.09 |
| 15.71 | 0.99 | 2.76 |
| 15.74 | 0.98 | 1.73 |
| 15.76 | 0.69 | 1.34 |
| 15.78 | 0.55 | 0.62 |
| 15.81 | 0.19 | 0.60 |
| 15.83 | 0.06 | 0.18 |
| 15.86 | 0.11 | 0.00 |
| 15.88 | 0.24 | -0.21 |
| 15.90 | 0.23 | 0.04 |
| 15.93 | -0.09 | -0.18 |
| 15.95 | 0.07 | -0.17 |
| 15.98 | 0.19 | -0.32 |
| 16.00 | 0.09 | 0.11 |
| 16.02 | 0.15 | -0.37 |
| 16.05 | -0.01 | 0.15 |
| 16.07 | 0.19 | -0.01 |
| 16.10 | -0.03 | 0.12 |
| 16.12 | 0.04 | -0.05 |
| 16.14 | -0.18 | 0.17 |
| 16.17 | 0.05 | -0.07 |
| 16.19 | 0.18 | -0.07 |
| 16.22 | -0.06 | 0.26 |
| 16.24 | 0.16 | -0.02 |
| 16.26 | 0.20 | 0.04 |
| 16.29 | -0.14 | 0.11 |
| 16.31 | 0.16 | 0.35 |
| 16.34 | 0.12 | -0.13 |
| 16.36 | -0.08 | -0.06 |
| 16.38 | 0.16 | 0.20 |
| 16.41 | 0.04 | -0.11 |
| 16.43 | 0.14 | -0.11 |
| 16.46 | 0.04 | -0.11 |
| 16.48 | -0.08 | 0.08 |
| 16.50 | 0.17 | 0.23 |
| 16.53 | 0.15 | 0.02 |
| 16.55 | -0.09 | 0.11 |
| 16.58 | 0.13 | -0.38 |
| 16.60 | -0.07 | 0.02 |
| 16.62 | -0.07 | 0.03 |
| 16.65 | 0.11 | 0.03 |
| 16.67 | 0.10 | 0.07 |
| 16.70 | 0.04 | -0.08 |
| 16.72 | 0.13 | -0.07 |
| 16.74 | 0.02 | -0.05 |
| 16.77 | -0.03 | -0.01 |
| 16.79 | -0.01 | 0.14 |
| 16.82 | -0.18 | -0.27 |
| 16.84 | -0.04 | 0.01 |
| 16.86 | -0.19 | -0.28 |
| 16.89 | 0.00 | -0.01 |
| 16.91 | -0.20 | 0.17 |
| 16.94 | 0.11 | -0.07 |
| 16.96 | -0.02 | 0.03 |
| 16.98 | 0.19 | -0.02 |
| 17.01 | -0.19 | 0.01 |
| 17.03 | -0.19 | 0.15 |
| 17.06 | -0.10 | 0.08 |
| 17.08 | 0.14 | -0.07 |
| 17.11 | -0.17 | 0.01 |
| 17.13 | 0.05 | -0.09 |
| 17.15 | -0.21 | -0.22 |
| 17.18 | -0.06 | 0.31 |
| 17.20 | -0.08 | 0.22 |
| 17.23 | -0.06 | 0.07 |
| 17.25 | 0.21 | -0.19 |
| 17.27 | -0.14 | -0.08 |
| 17.30 | 0.10 | 0.01 |
| 17.32 | 0.05 | 0.02 |
| 17.35 | 0.08 | -0.02 |
| 17.37 | 0.07 | -0.07 |
| 17.39 | -0.08 | -0.16 |
| 17.42 | 0.19 | -0.11 |
| 17.44 | -0.16 | -0.21 |
| 17.47 | 0.14 | 0.21 |
| 17.49 | 0.05 | -0.04 |
| 17.51 | -0.14 | -0.05 |
| 17.54 | 0.17 | 0.28 |
| 17.56 | 0.19 | 0.25 |
| 17.59 | 0.20 | 0.17 |
| 17.61 | -0.15 | 0.22 |
| 17.63 | 0.19 | 0.18 |
| 17.66 | 0.07 | 0.22 |
| 17.68 | 0.16 | 0.26 |
| 17.71 | 0.16 | 0.05 |
| 17.73 | -0.05 | 0.32 |
| 17.75 | -0.05 | -0.05 |
| 17.78 | 0.00 | 0.03 |
| 17.80 | 0.02 | -0.10 |
| 17.83 | 0.12 | -0.15 |
| 17.85 | 0.15 | -0.03 |
| 17.87 | 0.07 | -0.34 |
| 17.90 | 0.10 | -0.01 |
| 17.92 | 0.00 | -0.10 |
| 17.95 | -0.18 | 0.23 |
| 17.97 | -0.07 | 0.29 |
| 17.99 | 0.00 | -0.19 |
| 18.02 | 0.01 | -0.06 |
| 18.04 | -0.16 | -0.07 |
| 18.07 | 0.00 | -0.16 |
| 18.09 | 0.08 | 0.20 |
| 18.11 | 0.18 | 0.22 |
| 18.14 | -0.05 | -0.05 |
| 18.16 | -0.09 | 0.37 |
| 18.19 | 0.00 | -0.04 |
| 18.21 | 0.06 | 0.11 |
| 18.23 | -0.17 | -0.18 |
| 18.26 | 0.08 | -0.05 |
| 18.28 | 0.03 | -0.23 |
| 18.31 | 0.09 | -0.07 |
| 18.33 | -0.10 | -0.20 |
| 18.35 | -0.14 | -0.02 |
| 18.38 | -0.13 | 0.35 |
| 18.40 | 0.03 | 0.05 |
| 18.43 | -0.03 | 0.19 |
| 18.45 | -0.11 | -0.16 |
| 18.47 | 0.12 | -0.26 |
| 18.50 | -0.03 | -0.35 |
| 18.52 | 0.15 | -0.03 |
| 18.55 | 0.06 | -0.03 |
| 18.57 | -0.18 | -0.15 |
| 18.59 | 0.11 | 0.13 |
| 18.62 | 0.12 | 0.12 |
| 18.64 | 0.02 | 0.21 |
| 18.67 | -0.04 | -0.19 |
| 18.69 | -0.09 | -0.01 |
| 18.71 | 0.10 | -0.21 |
| 18.74 | 0.04 | 0.01 |
| 18.76 | 0.06 | 0.17 |
| 18.79 | -0.03 | 0.03 |
| 18.81 | -0.05 | -0.27 |
| 18.83 | 0.09 | -0.03 |
| 18.86 | 0.08 | -0.30 |
| 18.88 | -0.19 | 0.01 |
| 18.91 | 0.20 | -0.12 |
| 18.93 | 0.09 | 0.20 |
| 18.95 | -0.05 | -0.27 |
| 18.98 | 0.16 | 0.19 |
| 19.00 | -0.15 | 0.18 |
| 19.03 | 0.19 | -0.15 |
| 19.05 | -0.16 | 0.21 |
| 19.08 | 0.10 | 0.01 |
| 19.10 | -0.17 | 0.04 |
| 19.12 | -0.06 | -0.13 |
| 19.15 | 0.03 | -0.17 |
| 19.17 | -0.13 | 0.16 |
| 19.20 | -0.08 | 0.01 |
| 19.22 | -0.07 | 0.02 |
| 19.24 | -0.03 | 0.08 |
| 19.27 | 0.01 | 0.22 |
| 19.29 | 0.07 | 0.17 |
| 19.32 | 0.03 | 0.19 |
| 19.34 | -0.18 | -0.30 |
| 19.36 | 0.12 | 0.23 |
| 19.39 | 0.14 | 0.06 |
| 19.41 | 0.01 | -0.10 |
| 19.44 | -0.10 | -0.33 |
| 19.46 | 0.13 | -0.05 |
| 19.48 | 0.00 | 0.11 |
| 19.51 | 0.19 | -0.15 |
| 19.53 | -0.12 | 0.17 |
| 19.56 | 0.14 | 0.10 |
| 19.58 | -0.06 | -0.13 |
| 19.60 | -0.14 | -0.17 |
| 19.63 | 0.00 | 0.15 |
| 19.65 | 0.10 | 0.25 |
| 19.68 | 0.02 | 0.02 |
| 19.70 | 0.09 | 0.30 |
| 19.72 | -0.03 | 0.01 |
| 19.75 | -0.13 | -0.16 |
| 19.77 | -0.17 | 0.19 |
| 19.80 | -0.06 | -0.09 |
| 19.82 | -0.14 | 0.12 |
| 19.84 | 0.06 | 0.24 |
| 19.87 | 0.01 | 0.25 |
| 19.89 | 0.02 | -0.18 |
| 19.92 | -0.16 | 0.25 |
| 19.94 | -0.12 | -0.16 |
| 19.96 | 0.15 | 0.12 |
| 19.99 | -0.04 | -0.25 |
| 20.01 | 0.16 | -0.20 |
| 20.04 | 0.05 | -0.15 |
| 20.06 | -0.06 | 0.15 |
| 20.08 | -0.05 | -0.03 |
| 20.11 | 0.07 | -0.18 |
| 20.13 | -0.18 | 0.01 |
| 20.16 | 0.08 | 0.00 |
| 20.18 | 0.03 | 0.24 |
| 20.20 | -0.10 | -0.32 |
| 20.23 | 0.02 | -0.12 |
| 20.25 | -0.08 | 0.03 |
| 20.28 | -0.05 | -0.08 |
| 20.30 | -0.09 | 0.12 |
| 20.32 | -0.04 | 0.02 |
| 20.35 | -0.15 | 0.09 |
| 20.37 | 0.10 | -0.23 |
| 20.40 | 0.21 | -0.29 |
| 20.42 | -0.16 | -0.07 |
| 20.44 | -0.02 | 0.00 |
| 20.47 | -0.19 | -0.30 |
| 20.49 | 0.11 | -0.17 |
| 20.52 | 0.03 | -0.08 |
| 20.54 | 0.19 | 0.00 |
| 20.56 | -0.16 | 0.13 |
| 20.59 | 0.14 | 0.07 |
| 20.61 | 0.15 | 0.08 |
| 20.64 | 0.15 | -0.10 |
| 20.66 | 0.07 | 0.06 |
| 20.68 | 0.16 | -0.06 |
| 20.71 | 0.03 | 0.10 |
| 20.73 | -0.09 | 0.16 |
| 20.76 | 0.21 | 0.17 |
| 20.78 | 0.06 | -0.30 |
| 20.80 | 0.12 | 0.23 |
| 20.83 | -0.09 | -0.20 |
| 20.85 | -0.09 | 0.07 |
| 20.88 | 0.07 | 0.01 |
| 20.90 | -0.10 | 0.23 |
| 20.92 | -0.01 | 0.13 |
| 20.95 | -0.05 | 0.03 |
| 20.97 | 0.09 | 0.02 |
| 21.00 | 0.14 | 0.14 |
| 21.02 | 0.01 | 0.28 |
| 21.05 | 0.03 | -0.12 |
| 21.07 | -0.01 | -0.13 |
| 21.09 | -0.06 | -0.08 |
| 21.12 | 0.06 | 0.21 |
| 21.14 | 0.15 | -0.08 |
| 21.17 | -0.01 | -0.25 |
| 21.19 | 0.01 | -0.12 |
| 21.21 | 0.15 | 0.32 |
| 21.24 | -0.10 | 0.07 |
| 21.26 | 0.05 | 0.21 |
| 21.29 | -0.01 | 0.03 |
| 21.31 | -0.10 | 0.10 |
| 21.33 | 0.11 | 0.12 |
| 21.36 | -0.08 | -0.03 |
| 21.38 | -0.11 | -0.13 |
| 21.41 | -0.15 | -0.21 |
| 21.43 | -0.09 | 0.01 |
| 21.45 | 0.16 | -0.08 |
| 21.48 | -0.10 | -0.10 |
| 21.50 | -0.01 | -0.06 |
| 21.53 | -0.16 | -0.13 |
| 21.55 | -0.19 | 0.03 |
| 21.57 | -0.06 | 0.16 |
| 21.60 | -0.04 | 0.05 |
| 21.62 | -0.14 | -0.21 |
| 21.65 | 0.10 | -0.01 |
| 21.67 | -0.13 | -0.14 |
| 21.69 | -0.14 | 0.12 |
| 21.72 | 0.11 | -0.19 |
| 21.74 | -0.01 | -0.04 |
| 21.77 | -0.01 | 0.04 |
| 21.79 | -0.01 | 0.22 |
| 21.81 | -0.03 | 0.31 |
| 21.84 | 0.14 | 0.03 |
| 21.86 | -0.04 | -0.03 |
| 21.89 | -0.07 | 0.01 |
| 21.91 | -0.16 | 0.29 |
| 21.93 | -0.08 | -0.15 |
| 21.96 | -0.13 | -0.03 |
| 21.98 | -0.19 | 0.18 |
| 22.01 | 0.17 | -0.30 |
| 22.03 | 0.09 | 0.27 |
| 22.05 | 0.14 | -0.35 |
| 22.08 | -0.18 | -0.05 |
| 22.10 | 0.09 | 0.01 |
| 22.13 | -0.17 | 0.13 |
| 22.15 | -0.03 | 0.16 |
| 22.17 | -0.15 | 0.10 |
| 22.20 | 0.11 | 0.11 |
| 22.22 | 0.12 | 0.05 |
| 22.25 | -0.14 | 0.03 |
| 22.27 | 0.16 | 0.24 |
| 22.29 | -0.09 | -0.11 |
| 22.32 | -0.02 | -0.23 |
| 22.34 | 0.21 | -0.14 |
| 22.37 | -0.17 | -0.12 |
| 22.39 | 0.06 | -0.01 |
| 22.41 | 0.16 | 0.13 |
| 22.44 | -0.16 | 0.25 |
| 22.46 | -0.09 | 0.14 |
| 22.49 | 0.06 | -0.28 |
| 22.51 | 0.10 | 0.23 |
| 22.53 | -0.18 | -0.21 |
| 22.56 | 0.17 | -0.26 |
| 22.58 | -0.15 | 0.07 |
| 22.61 | 0.02 | 0.20 |
| 22.63 | -0.20 | -0.18 |
| 22.65 | 0.08 | 0.00 |
| 22.68 | -0.20 | -0.27 |
| 22.70 | -0.05 | 0.20 |
| 22.73 | -0.07 | -0.19 |
| 22.75 | -0.05 | -0.10 |
| 22.77 | 0.02 | 0.17 |
| 22.80 | 0.11 | -0.08 |
| 22.82 | 0.10 | 0.04 |
| 22.85 | -0.03 | -0.11 |
| 22.87 | -0.05 | 0.03 |
| 22.89 | -0.19 | 0.15 |
| 22.92 | -0.12 | -0.01 |
| 22.94 | 0.16 | -0.07 |
| 22.97 | -0.04 | -0.32 |
| 22.99 | -0.13 | 0.21 |
| 23.02 | 0.06 | -0.23 |
| 23.04 | -0.10 | -0.30 |
| 23.06 | 0.09 | -0.22 |
| 23.09 | 0.05 | -0.33 |
| 23.11 | -0.09 | -0.21 |
| 23.14 | -0.14 | -0.03 |
| 23.16 | -0.17 | -0.06 |
| 23.18 | 0.15 | -0.20 |
| 23.21 | 0.04 | 0.05 |
| 23.23 | 0.09 | -0.34 |
| 23.26 | 0.19 | 0.25 |
| 23.28 | 0.20 | -0.10 |
| 23.30 | 0.16 | -0.18 |
| 23.33 | 0.05 | -0.03 |
| 23.35 | 0.06 | -0.08 |
| 23.38 | -0.17 | 0.19 |
| 23.40 | -0.09 | 0.17 |
| 23.42 | 0.03 | 0.35 |
| 23.45 | 0.14 | 0.06 |
| 23.47 | -0.09 | 0.17 |
| 23.50 | -0.02 | -0.25 |
| 23.52 | -0.04 | 0.03 |
| 23.54 | 0.01 | 0.00 |
| 23.57 | -0.11 | -0.10 |
| 23.59 | -0.02 | 0.15 |
| 23.62 | -0.20 | 0.15 |
| 23.64 | -0.11 | -0.16 |
| 23.66 | 0.13 | 0.12 |
| 23.69 | 0.17 | -0.14 |
| 23.71 | -0.13 | -0.26 |
| 23.74 | 0.19 | 0.09 |
| 23.76 | 0.11 | -0.04 |
| 23.78 | -0.16 | 0.32 |
| 23.81 | 0.19 | 0.05 |
| 23.83 | -0.14 | 0.21 |
| 23.86 | -0.05 | 0.13 |
| 23.88 | 0.15 | -0.09 |
| 23.90 | 0.17 | 0.21 |
| 23.93 | -0.17 | 0.13 |
| 23.95 | -0.10 | -0.07 |
| 23.98 | -0.11 | 0.21 |
| 24.00 | -0.05 | 0.02 |
This Document: 39
README: 50
Total: 89
Report made with rmarkdown (Allaire, Xie, McPherson, et al., 2021; Xie, Allaire, and Grolemund, 2018; Xie, Dervieux, and Riederer, 2020), RefManageR(McLean, 2020).
Theme is Minty by Bootswatch.
5 Bibliography
Packages
Allaire, J., Y. Xie, J. McPherson, et al. (2021). rmarkdown: Dynamic Documents for R. R package version 2.11. URL: https://CRAN.R-project.org/package=rmarkdown.
McLean, M. W. (2020). RefManageR: Straightforward BibTeX and BibLaTeX Bibliography Management. R package version 1.3.0. URL: https://github.com/ropensci/RefManageR/.
Xie, Y., J. Allaire, and G. Grolemund (2018). R Markdown: The Definitive Guide. ISBN 9781138359338. Boca Raton, Florida: Chapman and Hall/CRC. URL: https://bookdown.org/yihui/rmarkdown.
Xie, Y., C. Dervieux, and E. Riederer (2020). R Markdown Cookbook. ISBN 9780367563837. Boca Raton, Florida: Chapman and Hall/CRC. URL: https://bookdown.org/yihui/rmarkdown-cookbook.
References
d’Adda di Fagagna, F., G. R. Weller, A. J. Doherty, et al. (2003). “The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku”. En. In: EMBO reports 4.1, pp. 47-52.
Fuller, J. R. and P. A. Rice (2017). “Target DNA bending by the Mu transpososome promotes careful transposition and prevents its reversal”. In: eLife 6.
Gyles, C. and P. Boerlin (2014). “Horizontally transferred genetic elements and their role in pathogenesis of bacterial disease”. En. In: Veterinary pathology 51.2, pp. 328-340.
Harshey, R. M. (2012). “The Mu story: how a maverick phage moved the field forward”. In: Mobile DNA 3.1.
Shapiro, J. A. (1979). “Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements”. En. In: Proceedings of the National Academy of Sciences of the United States of America 76.4, pp. 1933-1937.